Control reactions were done without the addition of dna to rule out contaminations during pcr. By selecting the generuler dna ladder of interest in table 1, you will be directed to the corresponding product page for more details and ordering information. The 1kb dna step ladder has ten bluntended dna fragments ranging from 1kb to 10kb in 1kb increments. Promega corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, highvalue products for the life sciences.
Cluster analysis based on similarity was performed by the neighborjoining. Hyperladder 1kb is our most popular molecular weight marker, composed of a restriction digest plus one or more pcr products, especially designed for easy size determination of linear doublestranded dna fragments on 1% to 2% tae or tbe agarose gels. In a sterile, nucleasefree microcentrifuge tube, combine the following on ice. The wizard genomic dna purification kit is designed for isolation of dna from white blood cells, tissue culture cells and animal tissue,plant tissue, yeast, grampositive and gramnegative bacteria. These standards contain predetermined fragment or protein sizes and concentrations. Thermo scientific generuler 1 kb dna ladder is designed for sizing and approximate quantification of wide range doublestranded dna on agarose gel. Generuler dna ladders are ideal for dna sizing and approximate quantification of a wide range of doublestranded dna. The 5x green gotaq reaction buffer contains two dyes blue and yellow that separate during electrophoresis to monitor migration progress. Plus dna ladder if you need size markers for fragments between 100 bp and 12 kb. Im doing dna extraction using chelex and before dna purification, it have 260280 ratio start from 1, 1 1,4. Promega 1kb dna ladder molecular weight marker 1kb dna. Wizard genomic dna purification kit protocol promega.
The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. Thirtythree bluntended dna fragments ranging from 200bp to 6,600bp in 200bp increments. The generuler dna ladders can offer sharp peaks during capillary electrophoresis, for exceptional purity of dna fragments without additional dna fragments or dntps. Promega 1kb dna ladder molecular weight marker 1kb dna ladder.
The 1kb dna ladder has thirteen bluntended fragments with sizes ranging from 250bp to 10,000bp. Agarose gel analysis shows that the bands between 100 bp and 1,500 bp are accurate in size and distinguishable. Neb also offers a quickload version of this ladder. The 5x green gotaq reaction buffer is tested to ensure that it does not interfere with dna migration when it is used as a loading dye for agarose gel electrophoresis. Two internal features facilitate band identification. The 1 kb, 3 kb, and 6 kb bands contain more dna to provide internal orientation. For optimal resolution, the recommended agarose gel concentration is 0. Promega dna ladder molecular weight markers, 1kb dna. The bands between 400bp and bp are brighter than the dna ladder i have previously used which is a bonus as this is the range most of my dna. The approximate mass of dna in each of the bands is provided assuming a 0. Gtg 5 pcr is useful in differentiation of unknown bacterial isolates and 16s rrna analysis species identity of the bacteria in sarawak aquaculture environment. The dna ladder consists of proprietary plasmids which are. The benchtop 1 kb dna ladder promega, madison, wi, usa was used as a. Promega manufacturing and delivery systems continue to be fully operational during the covid19 outbreak.
Promega dna ladder molecular weight markers microbiology. The first lane of the gel is labeled input and contains the 1kb dna ladder without size selection. The ladders are not intended for use in quantitative analysis. Each ladder is provided with a tube of 6x blueorange loading dye.
The ladder consists of bands that are generated from pcr and restriction enzyme digestion of double stranded dna. Dna ladder is commonly used to determine the size of dna fragments by electrophoresis in routine molecular biology laboratories. Promega corporation 2800 woods hollow road madison, wi 537115399 usa telephone 6082744330 toll free 8003569526 fax 6082772516 internet. How can i prepare working solution with 1 kb dna ladder. All bands except the 800bp band are of equal intensity. The quickload purple 1 kb dna ladder is not intended for precise quantification of dna mass but can be used for approximating the mass of dna in comparably intense samples of similar size. Gtg pcr analysis and 16s rrna sequencing of bacteria from. Dna step ladders contain dna bands of defined sizes and equal intensity with fragment lengths at defined increments. Comes supplied with 1 vial of gel loading dye, purple 6x, no sds.
Dna electrophoersis ladder very blurry electrophoresis. Straightforward procedure for laboratory production of dna ladder article pdf available in journal of nucleic acids 20123. The 1 kb and 3 kb bands contain more dna to provide internal orientation. This only happened after i made the most recent aliquot, although i have tried to make a new aliquot since. Genotypic characterization of psittacid herpesvirus isolates from brazil. Promega 1kb dna step ladder molecular weight marker for. Generuler 1 kb dna ladder thermo fisher scientific.
A thermo scientific generuler 100 bp dna ladder red and promega 100 bp dna ladder blue. Dna mass ladders are specifically created for quantitative estimation of dna mass in gels. Eleven fragments that range in size from 100bp to 1,000bp in 100bp increments plus a band at 1,500bp. This dna 1 kb ladder contains 11 fragments consisting of 500 bp repeats from 0. Source pcr products and doublestranded dna digested with appropriate restriction enzymes, are phenol extracted and equilibrated to 10 mm trishcl ph 8. The benchtop 1kb dna ladder has bluntended fragments with sizes ranging from 250bp to 10,000bp. Quickload 1 kb dna ladder is a premixed, readytoload molecular weight marker containing bromophenol blue as a tracking dye. Promega 1kb dna step ladder molecular weight marker for electrophoresis ten bluntended dna fragments ranging from 1kb to 10kb in 1kb increments. In this study, we report a new procedure to prepare a dna ladder that.
I have recently used the hyperladder 1kb dna ladder as it is a cheaper alternative to a similar dna ladder i have previously been using. The 1 kb dna ladder is suitable for sizing linear doublestranded dna fragments from 250 bp to 10 kb. Generuler 100 bp dna ladder 250 5 x 50 g for 500 applications, 0. The 500bp fragment is present at increased intensity for easy identification. Pdf straightforward procedure for laboratory production. Thirteen bluntended fragments with sizes ranging from 250bp to 10,000bp. The bands of the ladder each contain from 1 to 12 repeats of a 1018bp dna fragment. A highintensity 500bp band stands out at the lowest segment of the ladder 1kb. The ladder was run in duplicate and visualized with ethidium bromide. Straightforward procedure for laboratory production of dna.
Dna marker blue bp 1kb dna ladder readytouse dna marker bp 1kb dna ladder is designed for use as molecular weight standard for agarose gel electrophoresis features. In addition to these 12 bands, the ladder contains vector dna fragments that range from 75 to 1636 bp. Product1kb dna step ladder, 90ug product codeg6941 substance number description amount symbols g190 blueorange 6x loading dye 1 g694 1kb dna step ladder 1 pagina. The approximate mass of dna in each band is provided 0. Our teams are in regular contact with suppliers and distributors worldwide and are taking all steps necessary to address both demands for diagnostic tools and reliable delivery of all products as quickly as possible. Sigmas 1kb ladder is provided in a solution of 10 mm trishcl ph 8. Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15000 bp. Dna ladders have defined sizes, and are not intended for use in quantitative analysis.
Invitrogen egel 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15,000 bp. Generuler 100 bp dna ladder thermo fisher scientific. Promega corporation 2800 woods hollow road madison, wi 537115399 usa telephone 6082744330 toll free 8003569526 fax 608 27 51 internet. Usually after dna purification, 260280 ratio will ranging between 1,82 pure dna but. Genotypic characterization of psittacid herpesvirus isolates. Solis biodyne 100 bp dna ladder ready to load is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 3,000 bp. For the convenience, the amounts of the 500 and 1,000 bp fragments are adjusted to show greater intensities100 bp dna ladder marker contains 11 double stranded dna fragments from 100bp to 1kb of 100bp increment and 1.
The 1 kb dna ladder is a unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to yield fragments, suitable for use as molecular weight standards for electrophoresis. The 100bp dna ladder has eleven fragments that range in size from 100bp to 1,000bp in 100bp increments with an additional band at 1,500bp. Gotaqr flexi dna polymerase product information sheet 9pim829. View more information about our dna stains technology. A case of natural coinfection of tilapia lake virus and aeromonas. Promega pcr product we will send a replacement or refund your account.
Gtg pcr analysis and 16s rrna sequencing of bacteria. The ladder is composed of fourteen chromatographypurified individual dna in base pairs. The dna step ladders are ladders of defined sizes with exact incremental steps between bands. These ladders consist of equimolar mixtures of dna fragments for determining the mass of unknown dna samples on gels in the low and high molecular weight ranges. Because rna migrates faster than dna through electrophoresis gels, the appropriate nucleic acid ladder rna vs dna should be used. Often, dna molecular weight markers and ladders come in two formats. Quickload purple 1 kb dna ladder is stable for at least 6 months at 25c.
The enzyme is a taq dna polymerase supplied in a proprietary formulation containing 50% glycerol with buffers designed for enhanced amplification. Promega dna ladder molecular weight markers, 100bp dna ladder. Promega products are warranted to meet or exceed the. The product is supplied in a solution of 10 mm trishcl, ph 8. Promega dna ladder molecular weight markers, 1kb dna ladder. Infection with anaplasma phagocytophilum in a seronegative patient. To the left of the gel is the 1kb dna ladder with its fragment sizes. For example, step ladder dna fragments could be in increments of 100bp from 100bp all the way up. The 500 bp band with higher concentration is distinguished easily from others. Promega corporation 2800 woods hollow road madison,wi 53711 u.
Egel 1 kb plus dna ladder consists of 18 individual chromatographypurified dna. Egel 1 kb plus dna ladder thermo fisher scientific. The promega benchtop 1kb dna ladder was separated on both a 0. The 1,000bp and 3,000bp fragments have increased intensity relative to the other bands on ethidium bromidestained agarose gels for easy identification. The ladder is really clear with great definition between bands. The wizard genomic dna purification kit is based on a fourstep process.
Forty bluntended dna fragments ranging from 100bp to 4,000bp in 100bp increments. The first step in the purification procedure lyses the cells and the. To determine fragment sizes, rna is typically run in a denaturing electrophoresis gel. A highintensity 500bp band stands out at the lowest segment of the ladder. Gotaq dna polymerasea,b is a taq dna polymerase supplied. Molecular weight markers, or ladders, are a set of standards that are used for determining the approximate size of a protein or a nucleic acid fragment run on an electrophoresis gel. The ladder is composed of fourteen chromatographypurified individual dna fragments in base pairs. The ladder is composed of chromatographypurified dna fragments dissolved in te buffer 10 mm tris ph 7. Dna electrophoersis ladder very blurry nov022010 recently my dna reference ladder invitrogen 1kb plus has been giving me a very blurred smeary result, rather than the distinct bands i am used to and used to get. The key to accurate band analysis is to use the correct dna ladder for your particular application. Similarly to dna ladders, rna ladders contain rna fragments of varying sizes. To be the most responsive supplier of biological reagents and reagent systems used in research and applied technology applications worldwide. The concentration of 1kb dna ladder is 100 ugml 500ul five microlitres 500 ng of the 1 kb dna ladder are mixed with 1ul of blueorange 6x loading dye. Promega 1kb dna step ladder molecular weight marker for electrophoresis 1kb dna step ladder to 10,000.
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